It does, trust me. I have been at live sets on the dancefloor with Jules playing and suddenly everyone is turning round with the "what the f*** is this?" face and not really dancing. THAT, to me is loss of atmosphere and, like trust, takes a good 15 mins to get back.
Isn't it amazing as a DJ you can build, build, build,lovely - but one wrong move it just completely fu*** it, it's all wrong and you go back to the bar to slag it off with your mates... it's all such a shame and it happens so much!